STR基因位點由長度為3～7個堿基對的短串連重復(fù)序列組成，這些重復(fù)序列廣泛存在于人類基因組中，可作為高度多態(tài)性標(biāo)記，被稱為細(xì)胞的DNA指紋，其可通過PCR（聚合酶鏈?zhǔn)椒磻?yīng)）來檢測。STR基因座位上的等位基因可通過擴(kuò)增區(qū)域內(nèi)重復(fù)序列的拷貝數(shù)的不同來區(qū)分，在毛細(xì)管電泳分離之后可通過熒光檢測來識別。隨后通過一定的計算方法，即可根據(jù)所得的STR分型結(jié)果與專業(yè)的細(xì)胞STR數(shù)據(jù)庫比對從而推算出樣品所屬的細(xì)胞系或可能的交叉污染的細(xì)胞系名稱。（美國的ATCC 細(xì)胞庫、德國的DSMZ細(xì)胞庫以及日本的JCRB細(xì)胞庫等為STR 分型提供了各細(xì)胞株的數(shù)據(jù)供比對。）

實驗方案：

樣本要求：

細(xì)胞團(tuán)>106個，或一瓶活細(xì)胞，或凍存細(xì)胞>106個；或DNA濃度>50ng/μl，體積>20μl

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